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gpx2 cdna sequence  (Addgene inc)


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    Structured Review

    Addgene inc gpx2 cdna sequence
    Gpx2 Cdna Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gpx2+cdna+sequence/GPX2+(Plasmid+%2338796)/pm41484137-411-8-27
    Average 94 stars, based on 1 article reviews
    gpx2 cdna sequence - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Oxidative stress and GPX2 control pancreatic vs. non-pancreatic cell fate in human endoderm
    Article Snippet: To ligate the GPX2 PCR product with vector NEBuilder HiFi DNA Assembly Cloning Kit (NEB, USA) was used. .. The resulting Gateway pENTR 1 A plasmid containing the GPX2 cDNA sequence was subjected to an LR recombination reaction with plasmid PB-TA-ERP2 (gift from Dr. Knut Woltjen, Addgene, USA, 80477), leading to the acquisition of the final plasmid (pENTR-h GPX2 -FLAG plasmid) for establishing doxycycline-inducible GPX2 overexpression in HUES8-iCas9 and H1 hPSCs. .. Next, HUES8-iCas9 and H1 hPSCs were transfected with the pENTR-h GPX2 -FLAG plasmid and pCMV-hyPB plasmid (gift from Dr. Allan Bradley), carrying the transposase enzyme using the Lipofectamine 3000 kit (Thermo Fisher Scientific, Netherlands), following manufacturer’s instructions.

    Article Title: Oxidative stress and GPX2 control pancreatic vs. non-pancreatic cell fate in human endoderm.
    Article Snippet: To ligate the GPX2 PCR product with AR TI CL E IN P RE SS vector NEBuilder HiFi DNA Assembly Cloning Kit (NEB, USA) was used. .. The resulting Gateway pENTR 1A plasmid containing the GPX2 cDNA sequence was subjected to an LR recombination reaction with plasmid PB-TA-ERP2 110 (gift from Dr. Knut Woltjen, Addgene, USA, 80477), leading to the acquisition of the final plasmid (pENTR-hGPX2-FLAG plasmid) for establishing doxycycline-inducible GPX2 overexpression in HUES8-iCas9 and H1 hPSCs. .. Next, HUES8iCas9 and H1 hPSCs were transfected with the pENTR-hGPX2-FLAG plasmid and pCMV-hyPB plasmid 111 (gift from Dr. Allan Bradley), carrying transposase enzyme using Lipofectamine 3000 kit (Thermo Fisher Scientific, Netherlands), following manufacturer’s instructions.

    Sequencing:

    Article Title: Oxidative stress and GPX2 control pancreatic vs. non-pancreatic cell fate in human endoderm
    Article Snippet: To ligate the GPX2 PCR product with vector NEBuilder HiFi DNA Assembly Cloning Kit (NEB, USA) was used. .. The resulting Gateway pENTR 1 A plasmid containing the GPX2 cDNA sequence was subjected to an LR recombination reaction with plasmid PB-TA-ERP2 (gift from Dr. Knut Woltjen, Addgene, USA, 80477), leading to the acquisition of the final plasmid (pENTR-h GPX2 -FLAG plasmid) for establishing doxycycline-inducible GPX2 overexpression in HUES8-iCas9 and H1 hPSCs. .. Next, HUES8-iCas9 and H1 hPSCs were transfected with the pENTR-h GPX2 -FLAG plasmid and pCMV-hyPB plasmid (gift from Dr. Allan Bradley), carrying the transposase enzyme using the Lipofectamine 3000 kit (Thermo Fisher Scientific, Netherlands), following manufacturer’s instructions.

    Article Title: Oxidative stress and GPX2 control pancreatic vs. non-pancreatic cell fate in human endoderm.
    Article Snippet: To ligate the GPX2 PCR product with AR TI CL E IN P RE SS vector NEBuilder HiFi DNA Assembly Cloning Kit (NEB, USA) was used. .. The resulting Gateway pENTR 1A plasmid containing the GPX2 cDNA sequence was subjected to an LR recombination reaction with plasmid PB-TA-ERP2 110 (gift from Dr. Knut Woltjen, Addgene, USA, 80477), leading to the acquisition of the final plasmid (pENTR-hGPX2-FLAG plasmid) for establishing doxycycline-inducible GPX2 overexpression in HUES8-iCas9 and H1 hPSCs. .. Next, HUES8iCas9 and H1 hPSCs were transfected with the pENTR-hGPX2-FLAG plasmid and pCMV-hyPB plasmid 111 (gift from Dr. Allan Bradley), carrying transposase enzyme using Lipofectamine 3000 kit (Thermo Fisher Scientific, Netherlands), following manufacturer’s instructions.

    Over Expression:

    Article Title: Oxidative stress and GPX2 control pancreatic vs. non-pancreatic cell fate in human endoderm
    Article Snippet: To ligate the GPX2 PCR product with vector NEBuilder HiFi DNA Assembly Cloning Kit (NEB, USA) was used. .. The resulting Gateway pENTR 1 A plasmid containing the GPX2 cDNA sequence was subjected to an LR recombination reaction with plasmid PB-TA-ERP2 (gift from Dr. Knut Woltjen, Addgene, USA, 80477), leading to the acquisition of the final plasmid (pENTR-h GPX2 -FLAG plasmid) for establishing doxycycline-inducible GPX2 overexpression in HUES8-iCas9 and H1 hPSCs. .. Next, HUES8-iCas9 and H1 hPSCs were transfected with the pENTR-h GPX2 -FLAG plasmid and pCMV-hyPB plasmid (gift from Dr. Allan Bradley), carrying the transposase enzyme using the Lipofectamine 3000 kit (Thermo Fisher Scientific, Netherlands), following manufacturer’s instructions.

    Article Title: Oxidative stress and GPX2 control pancreatic vs. non-pancreatic cell fate in human endoderm.
    Article Snippet: To ligate the GPX2 PCR product with AR TI CL E IN P RE SS vector NEBuilder HiFi DNA Assembly Cloning Kit (NEB, USA) was used. .. The resulting Gateway pENTR 1A plasmid containing the GPX2 cDNA sequence was subjected to an LR recombination reaction with plasmid PB-TA-ERP2 110 (gift from Dr. Knut Woltjen, Addgene, USA, 80477), leading to the acquisition of the final plasmid (pENTR-hGPX2-FLAG plasmid) for establishing doxycycline-inducible GPX2 overexpression in HUES8-iCas9 and H1 hPSCs. .. Next, HUES8iCas9 and H1 hPSCs were transfected with the pENTR-hGPX2-FLAG plasmid and pCMV-hyPB plasmid 111 (gift from Dr. Allan Bradley), carrying transposase enzyme using Lipofectamine 3000 kit (Thermo Fisher Scientific, Netherlands), following manufacturer’s instructions.



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    Addgene inc gpx2 cdna sequence
    Gpx2 Cdna Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gpx2+cdna+sequence/GPX2+(Plasmid+%2338796)/pm41484137-411-8-27
    Average 94 stars, based on 1 article reviews
    gpx2 cdna sequence - by Bioz Stars, 2026-10
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    		  Buy from Supplier

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